Coding
srpR

Part:BBa_K1725060:Design

Designed by: Mhairi Davidson   Group: iGEM15_Glasgow   (2015-08-12)


SrpR repressor from Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed 3 Spe1 restriction sites to make it BioBrick compatible.


Source

Sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html

References